Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP Cell
cell line
LNCaP Cell
cell type
prostate cancer cell line
chip antibody
anti-BRD4 (Bethyl Laboratories)
geo_loc_name
missing
collection_date
missing

Sequenced DNA Library

library_name
GSM6404054
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were constructed using the SMARTer ThruPLEX DNA-Seq Prep Kit (Takara Bio USA).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
16424607
Reads aligned (%)
19.0
Duplicates removed (%)
23.4
Number of peaks
68 (qval < 1E-05)

hg19

Number of total reads
16424607
Reads aligned (%)
18.8
Duplicates removed (%)
24.4
Number of peaks
93 (qval < 1E-05)

Base call quality data from DBCLS SRA